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Applications of Single Cell RNA Sequencing
The technique of Single-Cell RNA sequencing (scRNA-Seq) has enabled unprecedented insight into the biology of individual cells and tissue complexity across a broad range of discovery and disease areas. Below are some of the application areas where the accessibility, precision, and sensitivity of our PIPseqTM technology can enable new research and discoveries.
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Single Cell RNA-seq for Cancer Research
Understanding the differences in gene transcription between healthy and diseased cells are fundamental to cancer pathology. scRNA-Seq has been a vital tool for understanding:
- Differential gene expression
- Novel cancer biomarkers
- Tumor heterogeneity
- Drug resistance
- Cancer immunotherapy
Single Cell RNA-seq for Immunology
The immune system is composed of a complex hierarchy of diverse cell types that work in concert to identify, target, and eliminate pathogens. scRNA-Seq allows for:
- Identification of previously unknown sub-populations of immune cells
- Understanding developmental and differentiation pathways in immune cell populations
- Evaluation of the roles and functions of immune cell populations
![](https://www.fluentbio.com/wp-content/uploads/2021/10/Applications-Immunology.jpg)
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Single Cell RNA-seq for Neuroscience
Neuronal tissues comprise a complex organization of diverse cell types that continuously reorganize and remodel throughout an organism’s lifetime. scRNA-Seq has become a valuable tool to catalog the true diversity of neuronal cell populations with unprecedented transcriptional sensitivity providing insight into:
- Neurodevelopmental and neurodegenerative diseases
- Previously unknown roles of specific neuronal populations
- The effect of neuro-immune cells on disease and development
![](https://www.fluentbio.com/wp-content/uploads/2024/05/Fluent_Biosciences_PIPseqV_T20.png)
Featured Product
PIPseq™ V T20
3′ Single Cell RNA Kit
- Profile up to 2o,000 single cells per sample for $900
- Includes sample processing + library preparation reagents
- Compatible with Illumina sequencing
- Access to user-friendly downstream analysis portal
Single Cell RNA-Seq Case Studies
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Case Study
Time-course Study of Intestinal Organoids
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Study: Evaluate therapeutic-induced intestinal organoid samples (2,000 cells) using the T2 kit
Findings: PIPseq enabled a series of complex time-course studies in a simple, cost-effective workflow
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Case Study
Mouse Brain Tissue Study
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Study: Evaluate dissociated cells from mouse brain; 12,789 cells captured (with T20 Kit)
Findings: PIPseq clearly resolves diverse cell populations without the clogging issues associated with microfluidics
Case Study
Immune Cell Diversity in PBMCs
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Study: Assess immune cell diversity in PBMC samples using the T100 Kit (65K cells captured)
Findings: PIPseq resolves rare PBMC subpopulations with low depth sequencing (~4,800 reads/cell)