Announcing PIPseq V, Fluent’s Newest Breakthrough in Single-Cell Analysis

PIPseq™ 4.0PLUS

3’ Single Cell RNA Kits

  • Profile up to 100,000 single cells per reaction (2 reactions/kit)
    • Includes all cell capture and library preparation reagents
    • Compatible with Illumina NGS instruments
    • Access to user-friendly data analysis software

*Please e-mail to obtain copies of SDS for Fluent products

Included in This Kit

The PIPseq™ 4.0PLUS 3′ Single Cell RNA Kits provide cell capture and library preparation reagents to generate sequencing-ready libraries for 3’ gene expression profiling starting from single cell suspensions in a convenient 2-day workflow. These libraries enable profiling of up to 100,000 individual cells per sample and are compatible with Illumina NGS sequencing instruments. 

Required one-time accessories are sold separately (PIPseq™ Single Cell RNA Starter Kit), and the purchase of this kit includes access to a data analysis software (PIPseeker for Data Analysis).

Key Benefits

  • Easy to implement (no complex instrumentation or consumables required)
  • Flexibility to process from 1 to 2 samples per kit 
  • Cost-effectively run large scale studies
  • Conveniently capture RNA at point of collection
  • User-friendly software for data analysis

Low Multiplet Rate

A 50:50 mixture of 2500 human (HEK 293T)  and mouse (NIH/3T3) cells were processed through PIPseq. mRNA expression clearly resolves human and mouse cells with a multiplet rate below 5%. 

Complex Cell Population Clearly Resolved

~65,000 cells from PBMCs (at ~4,800 reads/cell) were identified by PIPseq™ and resolved by UMAP analysis Major cell types were clearly detected.

Single Cell RNA Kit Application Areas

Single-cell RNA sequencing (scRNA-Seq) has enabled unprecedented insight into the biology of individual cells and tissue complexity across a broad range of discovery and disease areas. Below are some of the application areas where the the accessibility, precision, and sensitivity of our PIPseq technology can enable new research and discoveries




Infectious Disease

PIPseq™ 4.0PLUS 3” Single Cell RNA Kits

  • Profile up to 100,000 cells in a single reaction
  • Includes cell capture + library preparation reagents
  • Access to downstream analysis software

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