Setting Up for Experimental Success: Exploring the Importance of Pilot Studies

PIPseq™ T20 3’
Single Cell RNA Kit v4.0PLUS

  • Profile up to 20,000 single cells per reaction (4 reactions per kit )
  • Priced at $900 per sample*
    • Includes all cell capture and library preparation reagents
    • Compatible with Illumina NGS instruments
    • Access to user-friendly data analysis software

*Prices vary outside the United States.

Please e-mail support@fluentbio.com to obtain copies of SDS for Fluent products

Included in this Kit

The PIPseq™ T20 3′ Single Cell RNA Kits provide cell capture and library preparation reagents to generate sequencing-ready libraries for 3’ gene expression profiling starting from single cell suspensions in a convenient 2-day workflow. These libraries enable profiling of up to 20,000 individual cells per sample and are compatible with Illumina NGS sequencing instruments. 

Required one-time accessories are sold separately (PIPseq™ Single Cell RNA Starter Kit), and the purchase of this kit includes access to a data analysis software (PIPseeker for Data Analysis).

Key Benefits

  • Easy to implement (no complex instrumentation or consumables required)
  • Flexibility to process anywhere from 1 to 4 samples per kit 
  • Cost-effectively run complex tissue studies and clinical samples
  • Conveniently capture RNA at the point of collection
  • User-friendly bioinformatics software for data analysis

Complex Cell Population Clearly Resolved

~80,000 captured cells from breast tissue were processed through PIPseq™ and resolved by UMAP analysis.  Major cell types were clearly detected.

Low Multiplet Rate

A 50:50 mixture of 2500 human (HEK 293T)  and mouse (NIH/3T3) cells were processed through PIPseq. mRNA expression clearly resolves human and mouse cells with a multiplet rate below 7%. 

High Quality Transcripts Captured

Representative barcode rank plot shows that over 1000 cells of high quality transcripts were captured with the PIPseq Single Cell RNA Kit using a 50:50 human-mouse cell mixture (HEK 293T/NIH/3T3 cells).  

Select from the below resources to learn more about our protocols (user guides) or customer applications (posters).

Single Cell RNA Kit Application Areas

Single-cell RNA sequencing (scRNA-Seq) has enabled unprecedented insight into the biology of individual cells and tissue complexity across a broad range of discovery and disease areas. Below are some of the application areas where the the accessibility, precision, and sensitivity of our PIPseq technology can enable new research and discoveries

Cancer

Immunology

Neuroscience

Infectious Disease

PIPseq™ T20 3′ Single Cell RNA Kits

  • Profile up to 20,000 cells (T20), at $900/sample
  • Includes cell capture + library preparation reagents
  • Access to downstream analysis software

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