Fluent BioSciences has enhanced the PIPseq 3’ Single Cell RNA product line with the Supplemental Enrichment and Amplification (SEA) Kit that enables a diversity of custom applications using the PIPseq platform. PIPseq is unique among scRNA-Seq technologies in that the cDNA generated during reverse transcription remains bound to the Fluent hydrogel beads (bead-bound DNA templates) such that they can be reused in subsequent reactions after initial generation of the whole transcriptome.
The SEA Kit provides the reagents necessary for these supplemental amplifications. In addition, the SEA kit enables enrichment of selected molecules in the cDNA pool, including but not limited to, synthetic nucleotide tags (SNT), which the user introduces to label or modify select targets in a sample.
Select from the below resources to learn more about our protocols (user guides) or customer applications (posters).
Single Cell RNA Kit Application Areas
Single-cell RNA sequencing (scRNA-Seq) has enabled unprecedented insight into the biology of individual cells and tissue complexity across a broad range of discovery and disease areas. Below are some of the application areas where the the accessibility, precision, and sensitivity of our PIPseq technology can enable new research and discoveries
Supplemental Enrichment and Amplification (SEA) Kit:
- Supplemental amplification of bead-bound DNA templates
- Enrichment of synthetic nucleotide tags (SNTs) that were introduced to label select targets in a sample
- Enrichment of guide RNA tags that were introduced to modify cellular responses in select targets in a sample
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