Included in This Kit
The PIPseq™ T2 and T20 3′ Single Cell RNA Kits provide cell capture and library preparation reagents to generate sequencing-ready libraries for 3’ gene expression profiling starting from single cell suspensions in a convenient 2-day workflow. These libraries enable profiling of up to 2,000 (T2) or 20,000 (T20) individual cells per sample and are compatible with Illumina NGS sequencing instruments.
Required one-time accessories are sold separately (PIPseq™ Single Cell RNA Starter Kit), and the purchase of this kit includes access to a data analysis portal (Fluent Platform for Data Analysis).
- Easy to implement (no complex instrumentation or consumables required)
- Flexibility to process anywhere from 1 to 8 samples per kit
- Cost-effectively run pilot projects and optimization studies
- Conveniently process cells and capture RNA at point of collection
- User-friendly bioinformatics portal for data analysis
Low Multiplet Rate
A 50:50 mixture of 2500 human (HEK 293T) and mouse (NIH/3T3) cells were processed through PIPseq. mRNA expression clearly resolves human and mouse cells with a multiplet rate below 5%.
High quality transcripts captured
Representative barcode rank plot shows that over 1000 cells of high quality transcripts were captured with the PIPseq Single Cell RNA Kit using a 50:50 human-mouse cell mixture (HEK 293T/NIH/3T3 cells).
Complex cell population clearly resolved
~80,000 captured cells from breast tissue were processed through PIPseq™ and resolved by UMAP analysis. Major cell types were clearly detected.
Select from the below resources to learn more about our protocols (user guides) or customer applications (posters).
Single Cell RNA Kit Application Areas
Single-cell RNA sequencing (scRNA-Seq) has enabled unprecedented insight into the biology of individual cells and tissue complexity across a broad range of discovery and disease areas. Below are some of the application areas where the the accessibility, precision, and sensitivity of our PIPseq technology can enable new research and discoveries
PIPseq™ T2 and T20 3” Single Cell RNA Kits
- Profile up to 2,000 cells/sample (T2), or up to 20,000 cells (T20), starting at $300/sample
- Includes cell capture + library preparation reagents
- Access to downstream analysis portal
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