Press Release:  Fluent BioSciences expands access to industry’s most flexible and scalable technology (PIPseq) to enable single-cell analysis in any laboratory

PIPseq™ T2 and T20 3’
Single Cell RNA Kits

  • Two kit sizes (starting at $300 per sample)
    • T2: Profile up to 2,000 single cells per reaction (8 reactions per kit)
    • T20: Profile up to 20,000 single cells per reaction (4 reactions per kit)
  • Includes all cell capture and library preparation reagents
  • Compatible with Illumina NGS instruments
  • Access to user-friendly data analysis portal
  • NEW: Service lab option – easily capture cells with 1o minutes of hands-on time and ship to Fluent for processing!

Included in This Kit

The PIPseq™ T2 and T20 3′ Single Cell RNA Kits provide cell capture and library preparation reagents to generate sequencing-ready libraries for 3’ gene expression profiling starting from single cell suspensions in a convenient 2-day workflow. These libraries enable profiling of up to 2,000 (T2) or 20,000 (T20) individual cells per sample and are compatible with Illumina NGS sequencing instruments. 

Required one-time accessories are sold separately (PIPseq™ Single Cell RNA Starter Kit), and the purchase of this kit includes access to a data analysis portal (Fluent Platform for Data Analysis).

Key Benefits

  • Easy to implement (no complex instrumentation or consumables required)
  • Flexibility to process anywhere from 1 to 8 samples per kit 
  • Cost-effectively run pilot projects and optimization studies
  • Conveniently process cells and capture RNA at point of collection
  • User-friendly bioinformatics portal for data analysis

Low Multiplet Rate

A 50:50 mixture of 2500 human (HEK 293T)  and mouse (NIH/3T3) cells were processed through PIPseq. mRNA expression clearly resolves human and mouse cells with a multiplet rate below 5%. 

High quality transcripts captured

Representative barcode rank plot shows that over 1000 cells of high quality transcripts were captured with the PIPseq Single Cell RNA Kit using a 50:50 human-mouse cell mixture (HEK 293T/NIH/3T3 cells).  

Complex cell population clearly resolved

~80,000 captured cells from breast tissue were processed through PIPseq™ and resolved by UMAP analysis.  Major cell types were clearly detected.

Single Cell RNA Kit Application Areas

Single-cell RNA sequencing (scRNA-Seq) has enabled unprecedented insight into the biology of individual cells and tissue complexity across a broad range of discovery and disease areas. Below are some of the application areas where the the accessibility, precision, and sensitivity of our PIPseq technology can enable new research and discoveries

Cancer

Immunology

Neuroscience

Infectious Disease

PIPseq™ T2 and T20 3” Single Cell RNA Kits

  • Profile up to 2,000 cells/sample (T2), or up to 20,000 cells (T20), starting at $300/sample
  • Includes cell capture + library preparation reagents
  • Access to downstream analysis portal

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