PIPseq™ 3’
Single Cell RNA Kit

  • Cost effectively profile up to 2000 single cells per sample
  • Includes all cell capture and library preparation reagents
  • Compatible with Illumina NGS instruments
  • Access to user-friendly data analysis portal

Included in This Kit

The PIPseq™ Single Cell RNA Kit provides sample and library preparation reagents to generate sequencing-ready libraries for 3’ gene expression profiling starting from single cell suspensions in a convenient 2-day workflow. These libraries enable profiling of up to 2000 individual cells per sample and are compatible with Illumina NGS sequencing instruments. 

Required one-time accessories are sold separately (PIPseq™ Single Cell RNA Starter Kit), and the purchase of this kit includes access to a data analysis portal (Fluent Cloud Platform).

Key Benefits

  • Easy to implement (no complex instrumentation or consumables required)
  • Flexibility to process anywhere from 1 to 8 samples per kit 
  • Cost-effectively run pilot projects and optimization studies
  • Conveniently process cells and capture RNA at point of collection
  • User-friendly bioinformatics portal for data analysis

Low Multiplet Rate

A 50:50 mixture of 2500 human (HEK 293T)  and mouse (NIH/3T3) cells were processed through PIPseq. mRNA expression clearly resolves human and mouse cells with a multiplet rate below 5%. 

High quality transcripts captured

Representative barcode rank plot shows that over 1000 cells of high quality transcripts were captured with the PIPseq Single Cell RNA Kit using a 50:50 human-mouse cell mixture (HEK 293T/NIH/3T3 cells).  

Complex cell population clearly resolved

3000 captured PBMCs were processed through PIPseq™ and resolved by UMAP analysis.  Major immune cell populations were clearly detected.

Single Cell RNA Kit Application Areas

Single-cell RNA sequencing (scRNA-Seq) has enabled unprecedented insight into the biology of individual cells and tissue complexity across a broad range of discovery and disease areas. Below are some of the application areas where the the accessibility, precision, and sensitivity of our PIPseq technology can enable new research and discoveries

Cancer

Immunology

Neuroscience

Infectious Disease

PIPseq™ 3” Single Cell RNA Kit

  • Profile up to 2000 cells
  • Includes sample + library preparation reagents
  • Access to downstream analysis portal

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