Announcing PIPseq V, Fluent’s Newest Breakthrough in Single-Cell Analysis

PIPseq™ V T20
3’ Single Cell RNA Kit

  • Profile up to 20,000 single cells per reaction (4 reactions per kit )
  • Priced at $900 per sample*
    • Includes all cell capture and library preparation reagents
    • Compatible with Illumina NGS instruments
    • Access to user-friendly data analysis software

*Prices vary outside the United States.

Please e-mail support@fluentbio.com to obtain copies of SDS for Fluent products

Included in this Kit

The PIPseq™ V T20 3′ Single Cell RNA Kits provide cell capture and library preparation reagents to generate sequencing-ready libraries for 3’ gene expression profiling starting from single cell suspensions in a convenient 2-day workflow. These libraries enable profiling of up to 20,000 individual cells per sample and are compatible with Illumina NGS sequencing instruments. 

Required one-time accessories are sold separately (PIPseq™ Single Cell RNA Starter Kit), and the purchase of this kit includes access to a data analysis software (PIPseeker for Data Analysis).

Key Benefits

  • Easy to implement (no complex instrumentation or consumables required)
  • Flexibility to process anywhere from 1 to 4 samples per kit 
  • Cost-effectively run complex tissue studies and clinical samples
  • Conveniently capture RNA at the point of collection
  • User-friendly bioinformatics software for data analysis

Complex Cell Population Clearly Resolved

1M captured cells from mouse brain were processed through PIPseq and finely resolved cell populations were identified with low depth sequencing (2.7K reads/cell).

Low Multiplet Rate

A 50:50 mixture of human (HEK 293T) and mouse (NIH/3T3) were processed through PIPseq V. mRNA expression clearly resolves human and mouse cells with a multiplet rate below 2%.

High Quality Transcripts Captured

Representative barcode rank plot shows that high quality transcripts with low ambient RNA background were captured with the PIPseq V 3’ Single Cell RNA Kit using a 50:50 mixture of human (HEK 293T) and mouse (NIH/3T3). 

Single Cell RNA Kit Application Areas

Single-cell RNA sequencing (scRNA-Seq) has enabled unprecedented insight into the biology of individual cells and tissue complexity across a broad range of discovery and disease areas. Below are some of the application areas where the the accessibility, precision, and sensitivity of our PIPseq technology can enable new research and discoveries

Cancer

Immunology

Neuroscience

Infectious Disease

PIPseq™ V T20 3′ Single Cell RNA Kits

  • Profile up to 20,000 cells (T20), at $900/sample
  • Includes cell capture + library preparation reagents
  • Access to downstream analysis software

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