Announcing PIPseq V, Fluent’s Newest Breakthrough in Single-Cell Analysis

PIPseq™ V T2
3’ Single Cell RNA Kit

  • Profile up to 2,000 single cells per reaction (8 reactions per kit)
  • Priced at $300 per sample*
    • Includes all cell capture and library preparation reagents
    • Compatible with Illumina NGS instruments
    • Access to user-friendly data analysis software

*Prices vary outside the United States.

*Please e-mail support@fluentbio.com to obtain copies of SDS for Fluent products

Included in This Kit

The PIPseq™ V T2 3′ Single Cell RNA Kits provide cell capture and library preparation reagents to generate sequencing-ready libraries for 3’ gene expression profiling starting from single cell suspensions in a convenient 2-day workflow. These libraries enable profiling of up to 2,000 (T2) individual cells per sample and are compatible with Illumina NGS sequencing instruments. 

Required one-time accessories are sold separately (PIPseq™ Single Cell RNA Starter Kit), and the purchase of this kit includes access to a data analysis software (PIPseeker for Data Analysis).

Key Benefits

  • Easy to implement (no complex instrumentation or consumables required)
  • Flexibility to process anywhere from 1 to 8 samples per kit 
  • Cost-effectively run pilot projects and optimization studies
  • Conveniently process cells and capture RNA at point of collection
  • User-friendly bioinformatics portal for data analysis

Low Multiplet Rate

A 50:50 mixture of human (HEK 293T) and mouse (NIH/3T3) were processed through PIPseq V. mRNA expression clearly resolves human and mouse cells with a multiplet rate below 2%.

High Quality Transcripts Captured

Representative barcode rank plot shows that high quality transcripts with low ambient RNA background were captured with the PIPseq V 3’ Single Cell RNA Kit using a 50:50 mixture of human (HEK 293T) and mouse (NIH/3T3).  

Complex Cell Population Clearly Resolved

1M captured cells from mouse brain were processed through PIPseq and finely resolved cell populations were identified with low depth sequencing (2.7K reads/cell).

Single Cell RNA Kit Application Areas

Single-cell RNA sequencing (scRNA-Seq) has enabled unprecedented insight into the biology of individual cells and tissue complexity across a broad range of discovery and disease areas. Below are some of the application areas where the the accessibility, precision, and sensitivity of our PIPseq technology can enable new research and discoveries

Cancer

Immunology

Neuroscience

Infectious Disease

PIPseq™ V T2 3” Single Cell RNA Kits

  • Profile up to 2,000 cells/sample at $300/sample
  • Includes cell capture + library preparation reagents
  • Access to downstream analysis software

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