Datasets
Fluent BioSciences offers a variety of kits to address your single-cell experimental needs. Our example datasets include both a mouse/human cell mixture (“barnyard”) run, as well as immune cell profiling using PBMCs. Together, these datasets provide examples of our T2, T20 and T100 kit assay quality and ability to capture heterogeneity in complex cell mixtures.
Species Mixture Experiment with Large Cell Input
Barnyard Study (Human HEK 293T and Mouse NIH-3T3)
This dataset will be of particular relevance for researchers:
- Interested in the highest sensitivity PIPseq v4 chemistry performance
- Wanting to understand the impact of v4 chemistry improvements on heterotypic multiplet rates and other barnyard analysis outputs
Brain Nuclei (Mouse)
Nuclei from Frozen Mouse Brain
This dataset will be of particular relevance for researchers:
- You are interested in using PIPseq to perform single-nucleus RNA-seq.
- You want to assess the performance of PIPseq v4 with brain tissues.
Highly Scalable Immune Cell Profiling in a Single Reaction
Frozen PBMCs from a Healthy Donor
This dataset will be of particular relevance for researchers:
- Profiling heterogeneous cell populations at massive scale using the v4 chemistry
- Identifying very rare cell types
Species-Mixture Experiment (Human/Mouse)
1:1 Mixture of Human HEK293T and Mouse NIH-3T3 (~2.7k Cells)
This dataset will be of particular relevance for researchers:
- Interested in PIPseq v3 chemistry performance
- Profiling a limited number of cells
- Investigating heterotypic multiplet rates and other barnyard analysis outputs
Immune Cell Profiling using PBMCs
Frozen PBMCs from a Healthy Donor
This dataset will be of particular relevance for researchers:
- Interested in PIPseq v3 chemistry performance
- Profiling upwards of 20k cells in a heterogeneous cell population