PIPseq™ Epitope Sequencing Kit (Early Access)
- Enables multiplexed protein marker detection as well as cell hashing using polyadenylated oligonucleotide-tagged antibodies
- Use in combination with PIPseq T2, T20 or T100 3′ Single Cell RNA kit to profile the required number of single cells per reaction
- Validated with BioLegend TotalSeq™-A antibodies
Overview
The PIPseq™ Epitope Sequencing Kit uses Pre-templated Instant Partitions (PIPs) to combine antibody-stained cells with barcoded beads to produce sequencing-ready libraries starting from single cell suspensions in a convenient 2-day workflow. These libraries enable 3ʹ gene expression, multiplexed cellular protein measurements and sample multiplexing, and are compatible with Illumina NGS sequencing instruments.
Requires purchase of appropriate PIPseq 3′ Single Cell RNA Kit, antibodies and one-time accessories (PIPseq™ Single Cell RNA Starter Kit), which are are sold separately, and includes access to a data analysis software (PIPseeker for Data Analysis).
Related Resources
Select from the below resources to learn more about our protocols (user guides) or customer applications (posters).
Single Cell RNA Kit Application Areas
Single-cell RNA sequencing (scRNA-Seq) has enabled unprecedented insight into the biology of individual cells and tissue complexity across a broad range of discovery and disease areas. Below are some of the application areas where the the accessibility, precision, and sensitivity of our PIPseq technology can enable new research and discoveries
Cancer
Immunology
Neuroscience
Infectious Disease
PIPseq™ Epitope Sequencing Kit
- Enables multiplexed protein marker detection as well as cell hashing using polyadenylated oligonucleotide-tagged antibodies
- Use in combination with PIPseq T2, T20 or T100 3′ Single Cell RNA kit to profile the required number of single cells per reaction
- Validated with BioLegend TotalSeq-A antibodies
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