Fluent Service Lab
- Convenient full-service option to process your samples using our PIPseq technology
- Capture cells in any lab with 10 minutes of hands-on time
- Ship cells to Fluent for downstream processing
- Receive analyzed gene expression data back
- Process up to 20,000 captured cells/sample
- Flexible all-in pricing based on cells captured
The Fluent Service Lab option is well suited for evaluation or feasibility studies and provides an easy on-ramp to Fluent’s PIPseq single cell technology.
With this option, all you need to do is to capture cells with less than 10 minutes of hands-on time using the PIPseq T2 or T20 3′ Single Cell Capture and Lysis Kit, and then ship to the Fluent Service Lab in a pre-paid package. Our in-house experts will perform downstream processing from library prep & NGS through primary data analysis, providing QC reports and notifications along the way. The final data report includes basic cell metrics and clustering information, as well as feature-barcode matrices which are compatible with tertiary analysis software tools.
Workflow
Key Benefits
| Simple User Workflow | Prepare cell suspension, capture cells with Fluent kit (10 min hands-on time) and ship |
| Process at Fluent | Generate cDNA, prepare sequencing libraries, perform NGS and data analysis |
| Diverse Sample Types | Dissociated cells from solid tissue or cultured cells (<30 μm) |
| Flexible and Scalable | Process one or more samples from 500 to 20,000 cells/sample |
| Quick Turnaround Time | Primary analysis report in < 4 weeks |
| Highly Cost Effective | All-in-cost starting at $600/sample (500 cells) |
Case Study 1: Human Breast tissue Study
Objective: Resolve cell populations within a complex tissue in a simple, streamlined workflow.
Samples and # of cells: Dissociated cells from the breast; total of 82,000 cells captured across 4 samples
Key findings: PIPseq can resolve diverse cell populations from complex tissues.
Case Study 2: Mouse Brain tissue Study
Objective: Resolve cell populations within a complex tissue in a simple, streamlined workflow.
Samples and # of cells: Dissociated cells from mouse brain; total of 40,000 cells captured (in 2 samples)
Key findings: PIPseq resolves diverse cell populations from freshly dissociated brain tissue.
Case Study 3: Drug Response Study
Objective: Discriminate the transcriptomes of Gefitinib resistant and sensitive cell-lines after drug treatment
Samples and # of cells: Dissociated cells from the lung; ~500 cells captured
Key findings: PIPseq enables large-scale evaluation of treatment conditions at single cell resolution.
Related Resources
Select from the below resources to learn more about our protocols (user guides) or customer applications (posters).
Fluent Service Lab
- Convenient service lab option to process single cells
- Capture cells in any lab with 10 minutes of hands-on time
- Ship cells to Fluent for downstream processing
- Receive analyzed gene expression data back
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