Overcoming Common Challenges in Single Cell RNA-seq Experiments with PIPseq: UCSF Case Study

Fluent Platform for Data Analysis

  • Generate standard feature-barcode count matrices
  • Obtain summary metrics and diagnostic plots
  • Compatible with open-source secondary analysis packages

The Fluent Platform for Data Analysis enables primary analysis of PIPseq™ sequencing libraries.  The platform requires upload of your FASTQ files which are then processed into standard feature-barcode count matrices, along with summary metrics (such as genes or UMIs per cell) and diagnostic plots.

Count matrices are provided in a standard format compatible with widely-used open-source secondary analysis software packages.

Key Benefits

  • Works directly from FASTQ files

  • Processed into standard feature-barcode count matrices

  • Generate summary metrics (such as genes and UMIs per cell) and diagnostic plots

  • Count matrices are compatible with widely-used open-source secondary analysis software packages.