Accessible and highly scalable single-cell RNA-Seq solution for mRNA capture, barcoding, and library prep without complex workflows or microfluidics
Assay time
Hands-on time
Input quantity
Illumina Single Cell 3' RNA Prep kits make single-cell RNA sequencing (scRNA-Seq) accessible for more labs with an easy, scalable, and microfluidics-free workflow. Researchers can undertake larger and more varied multiomics studies without the budget or technology constraints of other single-cell approaches.
Illumina Single Cell 3' RNA Prep can easily scale with a wide processing range from hundreds to hundreds of thousands of cells. This increased cell throughput can reveal rare cell types. The assay is also flexible to run one sample or up to 96 samples in parallel with sample multiplexing.
Illumina Single Cell 3' RNA Prep kits offer high transcript and gene sensitivity with the high-quality data you expect from Illumina. The gentle isolation technique helps detect fragile cells that are often missed by other methods. The assay is compatible with custom single-cell measurements for expanded multiomic applications.
Illumina Single Cell 3' RNA Prep kits do not require expensive microfluidic equipment or labor-intensive protocols. You can conduct single-cell studies on your benchtop using a simple manual workflow with flexible stopping points.
Create templated emulsions using a vortex mixer to capture and barcode single-cell mRNA.
Generate cDNA and prepare single-cell libraries for sequencing.
Sequence on an NGS system to match the scale of your study.
Analyze and visualize single-cell data using DRAGEN and Partek software.
| Assay time | ~15 hr from cell capture to library prep |
|---|---|
| Content specifications | Captures the coding transcriptome |
| Description | Single-cell mRNA capture, barcoding, and library prep with a simple manual workflow, high transcript sensitivity, and cost-effective scalability. |
| Hands-on time | 10 mins for cell capture |
| Input quantity | 100 to 200,000 cells |
| Instruments | NextSeq 550 System, NextSeq 2000 System, NextSeq 1000 System, NextSeq 550Dx in Research Mode, NovaSeq X System, NovaSeq 6000Dx in Research Mode, NovaSeq 6000 System, NovaSeq X Plus System |
| Mechanism of action | PIPseq technology |
| Method | mRNA sequencing |
| Multiplexing | Up to 96-plex |
| Nucleic acid type | RNA |
| Specialized sample types | Blood, Not FFPE-compatible, Low-input samples, Bone marrow, Single cells |
| Species category | Any species, Mouse, Yeast, Zebrafish, Human |
| Strand specificity | Stranded |
| Technology | Sequencing |
| Variant class | Transcript variants |
Reagents for the NextSeq 1000/2000 System feature easy-to-use cartridges and multiple flow cell configurations for flexible sequencing options.
Reagent kits for the NovaSeq X Series include a reagent cartridge, buffer cartridge, flow cell, lyo insert, pre-load buffer, and library tube strip.
Reagent kits for the NovaSeq 6000 System consisting of ready-to-use pre-filled reagent cartridges and flow cells.
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